About the dataset

Background: In this set of dense-sampling, deep phenotyping studies, we determined whether day-to-day variation in sex hormone concentrations impacts large-scale brain network connectivity. In Study 1 (sessions 1-30, 2018), the female participant was naturally cycling; in Study 2 (sessions 31-60, 2019), the participant was placed on an oral hormonal contraceptive regimen.

Participant: The participant is a right-handed Caucasian female, aged 23 years for duration of the study 1. The participant had no history of neuropsychiatric diagnosis, endocrine disorders, or prior head trauma. She had a history of regular menstrual cycles (no missed periods, cycle occurring every 26-28 days) and had not taken hormone-based medication in the prior 12 months before the first study. The participant gave written informed consent and the study was approved by the University of California, Santa Barbara Human Subjects Committee.

Study Design: The participant underwent daily testing for 30 consecutive days. Each test session began with a daily questionnaire, followed by a time-locked collection of serum and whole blood started each day at 10:00am (±30 min), when the participant gave a blood sample. Endocrine samples were collected, at minimum, after two hours of no food or drink consumption (excluding water). The participant refrained from consuming caffeinated beverages before each test session. The MRI session lasted one hour and consisted of structural and functional MRI sequences.

Behavioral Assessments: To monitor state-dependent mood and lifestyle measures throughout the two studies, the following scales (adapted to reflect the past 24 hours) were administered each morning: Perceived Stress Scale (PSS; Cohen et al., 1983), Pittsburgh Sleep Quality Index (PSQI: Buysse et al., 1989), State-Trait Anxiety Inventory for Adults (STAI: Speillberger, 2010), and Profile of Mood States (POMS: Pollock et al., 1979). Behavioral data can be found under ‘participants.tsv’

Endocrine Procedures: A licensed phlebotomist inserted a saline-lock intravenous line into the dominant or non-dominant hand or forearm daily to evaluate hypothalamic-pituitary-gonadal axis hormones, including serum levels of gonadal hormones (17β-estradiol, progesterone and testosterone) and the pituitary gonadotropins luteinizing hormone (LH) and follicle stimulating hormone (FSH). One 10cc mL blood sample was collected in a vacutainer SST (BD Diagnostic Systems) each session. The sample clotted at room temperature for 45 min until centrifugation (2000 × g for 10 minutes) and were then aliquoted into three 1 mL microtubes. Serum samples were stored at -20°C until assayed. Serum concentrations were determined via liquid chromatography-mass spectrometry (for all steroid hormones) and immunoassay (for all gonadotropins) at the Brigham and Women’s Hospital Research Assay Core.

PI Emily Jacobs
Institution University of California, Santa Barbara
Study Website 28andMe, 28andMe-OpenNeuro
Data Descriptor Paper https://doi.org/10.1016/j.neuroimage.2020.117091
CUBIC Project open-datasets
DUA None
License CC BY-NC-SA
Sample Size 1
Number of Sessions 60
CuBIDS false
BABS false
Demographics View available demographics data
Average Age (y) 23.5
% Female 100
% Right Handed 100
BIDS Dataset
/cbica/projects/open-datasets/datasets/28andMe/dset
FreeSurfer v7.3.2
Run by fMRIPrep
(60/60 complete)
/cbica/projects/open-datasets/datasets/28andMe/derivatives/fmriprep/sourcedata/freesurfer
fMRIprep v25.0.0
(60/60 complete)
/cbica/projects/open-datasets/datasets/28andMe/derivatives/fmriprep


Funding
Brain and Behavior Research Foundation
California Nanosystems Institute
Institute for Collaborative Biotechnologies through US Army Research Office W911NF-19-D-0001
Rutherford B. Fett Fund

Citations
Pritschet L., Santander T., Taylor C., Layher E., Yu S., Miller M.B., Grafton S.T., Jacobs E.G. (2020) Functional reorganization of brain networks across the human menstrual cycle. NeuroImage.